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. Author manuscript; available in PMC: 2022 Apr 25.
Published in final edited form as: Methods Mol Biol. 2021;2245:195–213. doi: 10.1007/978-1-0716-1119-7_14

Fig. 3.

Fig. 3

Occurrence of mtDNA4977 deletion mutation in chondrocytes associated with SIRT3 deficiency. (a) Total DNA was directly isolated from human knee cartilage from normal and OA donors. PCR analyzed mtDNA4977 deletion mutation and total mtDNA as described in the Methods. Data in A represent primary chondrocytes from six normal and six OA donors. (b) Cell lysate SIRT3 expression was examined by Western blot analysis. Data in B represent five normal and five OA donors. (This figure is reproduced from ref. 13 with permission from Elsevier)