TABLE 2.
Oligonucleotide primers used for cloning, sequence determination, and mutation procedures
| Primer | Sequence | Restriction sitea |
|---|---|---|
| pneumo 1 | 5′-GATGAATGCATCGTGTCCATC-3′ | |
| pneumo 2 | 5′-CCGTCCGGTAGTTAGCAATCC-3′ | |
| pneumo 3 | 5′-CAAGGCACTCCAGCAGGCTCG-3′ | |
| pneumo 4 | 5′-CCCTGTCTCGCAGCGATACTAG-3′ | |
| pneumo 5 | 5′-CTGCTGGTGCCGATCTAGTC-3′ | |
| pneumo 6 | 5′-GTCAGACCAAAGCCAATTCC-3′ | |
| pneumo 7 | 5′-TGA GAA TTC ATG TCA AGT AAA GCC AAT-3′ | EcoRI |
| pneumo 8 | 5′-TGA TCT AGA TTA TTT ATA TTG TTT TAA-3′ | XbaI |
| pneumo-Δ | 5′-ACA ACA CGT TGG ATT TCC TCT TCT ATC TCA ATA TAA CTA C-3′ |
a
Sites of restriction enzyme cutting are underlined in the corresponding sequences.