Fig. 2. Representative plots from Muse® Oxidative Stress Assay. The above plots show the histograms of gated cells with two markers providing data on two cellular populations: marked with blue color: ROS(−) and marked with red color: ROS(+) cells. Cells were treated for 24 h with 1000 μg mL⁻¹ extract from non-enriched lettuce (control lettuce), or KIO₃-fortified lettuce, or 5-ISA-fortified lettuce, or 3,5-diISA-fortified lettuce, or synthetic 5-ISA, or synthetic 3,5-diISA, or 200 μM menadione as positive control.