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. 2022 Apr 25;17(4):e0266701. doi: 10.1371/journal.pone.0266701

Table 2. Antibody clones used for FACS analysis.

  Marker Fluorochrome Clone Manufacturer Cat. No.
Extracellular Staining Live/dead fixable Aqua - ThermoFisher L34965
CD3 VioBlue BW264/56 Miltenyi 170-081-046
CD4 APC VIT4 Miltenyi 130-113-210
CD8 PerCP BW135/80 Miltenyi 130-113-160
Intracellular Staining IFN-γ PE 45–15 Miltenyi 130-113-493
CD154 FITC 5C8 Miltenyi 130-113-606
TNF-α PE-Vio770 cA2 Miltenyi 130-120-492
IL-2 APC-Vio 770 REA689 Miltenyi 130-111-492