Table 2. Antibody clones used for FACS analysis.
Marker | Fluorochrome | Clone | Manufacturer | Cat. No. | |
---|---|---|---|---|---|
Extracellular Staining | Live/dead | fixable Aqua | - | ThermoFisher | L34965 |
CD3 | VioBlue | BW264/56 | Miltenyi | 170-081-046 | |
CD4 | APC | VIT4 | Miltenyi | 130-113-210 | |
CD8 | PerCP | BW135/80 | Miltenyi | 130-113-160 | |
Intracellular Staining | IFN-γ | PE | 45–15 | Miltenyi | 130-113-493 |
CD154 | FITC | 5C8 | Miltenyi | 130-113-606 | |
TNF-α | PE-Vio770 | cA2 | Miltenyi | 130-120-492 | |
IL-2 | APC-Vio 770 | REA689 | Miltenyi | 130-111-492 |