Fig 7. AP2L1 and AP2L5 genes modulate the flowering response in winter plants.
(A-E) Winter Kronos, winter UBIpro:miR172, winter ap2l1, winter ap2l5 and winter ap2l1 ap2l5 grown under LD conditions without vernalization (NV) and with 2 (2WV) weeks of vernalization. Box plots showing (A) days to heading or (B) number of leaves in the main tiller (n ≥ 6). (C-E) Expression levels of VRN1 (C), FT1 (D) and VRN2 (E) determined by qRT-PCR in the 9th leaves. After vernalization, the plants were moved to room temperature for 25 days before the fully expanded 9th leaf was collected. ACTIN was used as internal reference. Data correspond to four independent biological replicates. Different letters above the box plots indicate significant differences (P < 0.05) in Tukey tests, except for panels (B) 2WV and (D) NV where non-parametric Kruskal-Wallis tests were used (Data G in S1 Data).