Figure 5.

CCL5 deletion induces increased adaptive suppressor response and decreased CD8 T cell exhaustion. Adaptive immune cell populations were quantified from the tumor bearing lungs of sgCCL5, sgCNTRL and tumor free naïve. The sgCCL5 had higher CD19⁺ B cells, CD3⁺ CD8⁺ T cells, and no change to CD3⁺ CD4⁺ T cells when compared to sgCNTRL (a-c). In the CD4⁺ T cell populations, sgCCL5 had decreased CD25⁺ FOXP3⁺ (T_regs), decreased CD62L⁻ CD44⁺ (T_EM), and increased CD62L⁺ CD44⁻ (T_naive) compared to sgCNTRL (d-g). The CD8⁺ T cells in sgCCL5 had increased frequency of CD62L⁺ CD44⁺ (T_CM) and significantly decreased PD1^hi EOMES^hi TBET^lo CD8⁺ T_EM than the sgCNTRL (h-k). Data shown n = 8–12, bars indicate mean ± SEM, analyzed using an ANOVA with a post-hoc Tukey’s test. If data did not have normal distribution a Kruskal-Wallis test was performed. *P < .05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001