Figure 6.
High CCL5 transcriptomic expression in LUAD patients correlates with lower survival and suppression of effector response in tumors. (a) CIBERSORT analysis of BCCRC microarray data from 83 patients showing matched tumor and normal biopsies and cell subsets organized by -high to -low fold change tumor/normal (T/N) in the heatmap. (b) Frequency of Tregs in TCGA and BCCRC samples are significantly increased in tumor compared to normal tissues. (c) CIBERSORT Treg relative cell subset frequency in BCCRC LUAD samples plotted by stage of tumor development relative to matched normal lung tissue. (d) Violin plot showing CCL5 (RNAseq) expression separated by median (low (n = 251) and high (n = 251)) value for RAS-MAPK-ERK signature score in TCGA LUAD (see supplemental methods). (e) Bar graph of exhaustion linked genes in the CD8 T cells from CIBERSORTX for low (n = 127) and high (n = 127) tertiles of CCL5 expression in TCGA LUAD patients. (f) High expression of CCL5 (ID 1555759_a_at, split by median) was associated with poor outcomes in LUAD patients using the online program KM plotter. CIBERSORT is plotted as a box plot with whiskers representing min to max values. Significance for CIBERSORT, and RAS-MAPK-ERK signature score data by Mann-Whitney. Survival curves were compared using a log-rank (Mantel-cox) statistical test, *P < .05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001