Extended Data Fig. 6: In vitro and in vivo stability of Fc variants of anti-SARS-CoV-2 mAbs.
(a) Size-exclusion chromatography (SEC) analysis of Fc variants to determine aggregate formation among Fc domain variants. The SEC elution profiles and abundance (percentage) of monomeric IgG is indicated for the different Fc variants. (b) Fc variants of the REGN mAb cocktail were administered (i.v.; 50 μg) to FcγR humanized mice and antibody serum levels were determined by ELISA at various time points after antibody administration. n= total of 3 mice per group from two independent experiments. Data are mean ± s.e.m.