| Genetic reagent (D. melanogaster) |
E132Gal4: P{w[ + mW.hs] = GawB}E132, w[*] (also called upd-Gal4) |
Bloomington Drosophila Stock Center |
RRID:BDSC_26796
|
|
| Genetic reagent (D. melanogaster) |
TubGal80ts: w[*];P{w[ + mC] = tubP-GAL80[ts]}2/TM2 |
Bloomington Drosophila Stock Center |
RRID:DSC_7017
|
|
| Genetic reagent (D. melanogaster) |
UAS-GFP RNAi: w[1118]; P{w[ + mC] = UAS GFP.dsRNA.R}142 |
Bloomington Drosophila Stock Center |
RRID:BDSC_9330
|
|
| Genetic reagent (D. melanogaster) |
UAS-GFP RNAi: w[1118]; P{w[ + mC] = UAS GFP.dsRNA.R}143 |
Bloomington Drosophila Stock Center |
RRID:BDSC_9331
|
|
| Genetic reagent (D. melanogaster) |
UAS-Rbf RNAi: y1 sc[*] v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS03004}attP2/TM3, Sb1
|
Bloomington Drosophila Stock Center |
RRID:BDSC_36744
|
|
| Genetic reagent (D. melanogaster) |
UAS-Rbf RNAi: y1 sc[*] v1; P{y[ + t7.7] v[ + t1.8] = TRiP .GL01293}attP40/CyO |
Bloomington Drosophila Stock Center |
RRID:BDSC_41863
|
|
| Genetic reagent (D. melanogaster) |
C587Gal4: P{w[ + mW.hs] = GawB}C587, w[*] |
Bloomington Drosophila Stock Center |
RRID:BDSC_67747
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pointed.P1: w[1118]; P{w[ + mC] pnt[P1.UAS] = UAS pnt.P1}3 |
Bloomington Drosophila Stock Center |
RRID:BDSC_869
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pointed.P2: w[1118]; P{w[ + mC] pnt[P2.UAS] = UAS pnt.P2}2/TM3, Sb1
|
Bloomington Drosophila Stock Center |
RRID:BDSC_399
|
|
| Genetic reagent (D. melanogaster) |
UAS-Ras85D.V12: w[1118]; P{w[ + mC] = UAS-Ras85D.V12}TL1 |
Bloomington Drosophila Stock Center |
RRID:BDSC_4847
|
|
| Genetic reagent (D. melanogaster) |
UAS-Rolled: y1 w[*]; P{w[ + mC] = UAS-rl[Sem].S}2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_59006
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr Type I: w[*]; P{w[ + mC] = Egfr.1 .A887T.UAS}12–4/CyO, P{ry[ + t7.2] = sevRas1 .V12}FK1 |
Bloomington Drosophila Stock Center |
RRID:BDSC_9534
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr Type II: w[*]; P{w[ + mC] = Egfr.2 .A887T.UAS}8–2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_9533
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfrλ: w[*]; P{w[ + mC] = UAS Egfr.lambdatop}3/TM6C, Sb1
|
Bloomington Drosophila Stock Center |
RRID:BDSC_59843
|
|
| Genetic reagent (D. melanogaster) |
UAS-PVRλ: w[1118]; P{w[ + mC] = UASp Pvr.lambda}mP10 |
Bloomington Drosophila Stock Center |
RRID:BDSC_58496
|
|
| Genetic reagent (D. melanogaster) |
UAS-PVRλ: w[1118]; P{w[ + mC] = UASp Pvr.lambda}mP1 |
Bloomington Drosophila Stock Center |
RRID:BDSC_58428
|
|
| Genetic reagent (D. melanogaster) |
UAS-InR: y1 w[1118]; P{w[ + mC] = UAS InR.K414P}2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_8250
|
|
| Genetic reagent (D. melanogaster) |
UAS-InR: y1 w[1118]; P{w[ + mC] = UAS InR.A1325D}2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_8263
|
|
| Genetic reagent (D. melanogaster) |
UAS-Heartlessλ: y1 w[*]; P{w[ + mC] = UAS htl.lambda.M}40-22-2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_5367
|
|
| Genetic reagent (D. melanogaster) |
UAS-Breathlessλ: w[*]; P{w[ + mC] = UAS btl.lambda}2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_29045
|
|
| Genetic reagent (D. melanogaster) |
UAS-Sprouty RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01599}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_36709
|
|
| Genetic reagent (D. melanogaster) |
UAS-Pten RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00044}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_33643
|
|
| Genetic reagent (D. melanogaster) |
UAS-Argos RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.JF03020}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_28383
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr DN: y1 w[*]; P{w[ + mC] = UAS Egfr.DN.B}29-77-1; P{w[ + mC] = UAS Egfr.DN.B}29-8-1 |
Bloomington Drosophila Stock Center |
RRID:BDSC_5364
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.JF02283}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_36770
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS05003}attP40 |
Bloomington Drosophila Stock Center |
RRID:BDSC_60012
|
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr RNAi: w1118; P{GD1654}v43267 |
Vienna Drosophila Resource Center |
Stock #: 43,267 |
|
| Genetic reagent (D. melanogaster) |
UAS-Egfr RNAi: P{KK100051}VIE-260B |
Vienna Drosophila Resource Center |
Stock #: 107,130 |
|
| Genetic reagent (D. melanogaster) |
UAS-N-CA: P{ry[ + t7.2] = hsFLP}1, y1 w[*]; P{w[ + mC] = UAS N.intra.GS}2/CyO; MKRS/TM2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_52008
|
No longer available |
| Genetic reagent (D. melanogaster) |
UAS-N-DN: y[ + t7.2] = hsFLP}12, y1 w[*]; P{w[ + mC] = UAS N.ECN}2; MKRS/TM2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_51667
|
|
| Genetic reagent (D. melanogaster) |
UAS-Notch RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00001}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_33611
|
|
| Genetic reagent (D. melanogaster) |
UAS-Notch RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00009}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_33616
|
|
| Genetic reagent (D. melanogaster) |
UAS-Notch RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00092}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_35213
|
|
| Genetic reagent (D. melanogaster) |
UAS-Notch RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.GLV21004}attP2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_35620
|
|
| Genetic reagent (D. melanogaster) |
UAS-G-TRACE: w[*]; P{w[ + mC] = UAS-RedStinger}4, P{w[ + mC] = UAS FLP.D}JD1, P{w[ + mC] = Ubi-p63E(FRT.STOP)Stinger}9F6/CyO |
Bloomington Drosophila Stock Center |
RRID:BDSC_28280
|
|
| Genetic reagent (D. melanogaster) |
UAS-G-TRACE: w[*]; P{w[ + mC] = UAS-RedStinger}6, P{w[ + mC] = UAS FLP.Exel}3, P{w[ + mC] = Ubi-p63E(FRT.STOP)Stinger}15F2 |
Bloomington Drosophila Stock Center |
RRID:BDSC_28281
|
|
| Genetic reagent (D. melanogaster) |
UAS-secreted spitz: w[*]; P{w[ + mC] = UAS-sSpiCS}T28 |
Bloomington Drosophila Stock Center |
RRID:BDSC_63134
|
|
| Genetic reagent (D. melanogaster) |
UAS-secreted spitz: w[*]; P{w[ + mC] = UASp spi.sec}3/TM3, Ser1() |
Bloomington Drosophila Stock Center |
RRID:BDSC_58436
|
|
| Genetic reagent (D. melanogaster) |
UAS-secreted gurken: w[*]; P{w[ + mC] = UASp grk.sec}2/CyO |
Bloomington Drosophila Stock Center |
RRID:BDSC_58417
|
|
| Genetic reagent (D. melanogaster) |
UAS-gurken ΔTC |
PMID: 10559478 |
|
Dr. Trudi Schüpbach (Princeton University) |
| Genetic reagent (D. melanogaster) |
UAS- secreted Keren |
PMID:12169630 |
|
Dr. Matthew Freeman (MRC Laboratory of Molecular Biology) |
| Genetic reagent (D. melanogaster) |
UAS-Vein |
PMID:8824589 |
|
Dr. Amanda Simcox (The Ohio State University) |
| Genetic reagent (D. melanogaster) |
UAS-Grim |
PMID:9846179 |
|
Dr. John Nambu (University of Massachusetts) |
| Genetic reagent (D. melanogaster) |
Egfr(-): Egfr[f24]/T(2;3)TSTL, CyO: TM6B, Tb1
|
Bloomington Drosophila Stock Center |
RRID:BDSC_6500
|
|
| Antibody |
(Mouse monoclonal) anti–Fasciclin III (Drosophila) |
DSHB |
Cat#: 7G10; RRID: AB_528238
|
IHC (1:50) |
| Antibody |
(Mouse monoclonal) anti-phospho-Histone H3 (Ser10) (6G3) |
Cell Signaling Technology |
Cat#: 9,706 S; RRID:AB_331748
|
IHC (1:400) |
| Antibody |
(Guinea Pig polyclonal) anti-Traffic Jam |
Laboratory of D. Godt (Li et al., 2003) |
N/A |
IHC (1:20,000) |
| Antibody |
(Rabbit polyclonal) anti-Vasa (d-260) |
Santa Cruz Biotechnology |
Cat#: SC-30210; RRID:AB_793874
|
IHC (1:200) |
| Antibody |
(Chicken polyclonal) anti-GFP |
Abcam |
Cat#: ab13970; RRID:AB_300798
|
IHC (1:10,000) |
| Antibody |
(Rabbit polyclonal) anti-DsRed |
Takara Bio |
Cat#: 632496; RRID:AB_10013483
|
IHC (1:10,000) |
| Antibody |
(Rabbit polyclonal) anti-dpErk |
Cell Signaling Technology |
Cat#: 4370; RRID:AB_2315112
|
IHC (1:100) |
| Antibody |
(Goat polyclonal) anti-Mouse IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate |
ThermoFisher Scientific |
Cat#: A11029; RRID:AB_2534088
|
IHC (1:400) |
| Antibody |
(Goat polyclonal) anti-Rabbit IgG (H + L) secondary antibody, Alexa Fluor 568 conjugate |
ThermoFisher Scientific |
Cat#: A11011; RRID:AB_143157
|
IHC (1:200) |
| Antibody |
(Goat polyclonal) anti-Chicken IgY (H + L) secondary antibody, Alexa Fluor 488 conjugate |
ThermoFisher Scientific |
Cat#: A11039; RRID:AB_2534096
|
IHC (1:400) |
| Antibody |
(Goat polyclonal) anti-Guinea Pig IgG (H + L) secondary antibody, Alexa Fluor 568 conjugate |
ThermoFisher Scientific |
Cat#: A11075; RRID:AB_2534119
|
IHC (1:200) |
| Antibody |
(Goat polyclonal) anti-Guinea Pig IgG (H + L) secondary antibody, Alexa Fluor 633 conjugate |
ThermoFisher Scientific |
Cat#: A21105; RRID:AB_2535757
|
IHC (1:200) |
| Chemical compound, drug |
4,6-diamidino-2-phenylindole (DAPI) |
Millipore/Sigma (formerly Sigma-Aldrich) |
Cat#: 10236276001; CAS: 28718-90-3 |
IHC (1 μg/mL) |
| Chemical compound, drug |
16% Paraformaldehyde (formaldehyde) aqueous solution |
Electron Microscopy Sciences |
Cat#: 15710; CAS: 50-00-0 |
|
| Chemical compound, drug |
Goat Serum |
Millipore/Sigma (formerly Sigma-Aldrich) |
Cat#: G9023 |
|
| Chemical compound, drug |
Vectashield antifade mounting medium |
Vector Laboratories |
Cat#: H-1000 |
|
| Chemical compound, drug |
Phosphatase Inhibitor Cocktail 2 |
Millipore/Sigma (formerly Sigma-Aldrich) |
Cat#: P5726 |
|
| Chemical compound, drug |
DMSO (Dimethyl Sulfoxide), Sterile |
Cell Signaling Technology |
Cat#: 12,611 P |
|
| Software, algorithm |
Fiji |
Schindelin et al., 2012
|
https://www.fiji.sc/
|
|
| Software, algorithm |
Zeiss LSM |
Carl Zeiss Microscopy |
https://www.zeiss.com/microscopy/us/downloads/lsm-5-series.html
|
|
| Software, algorithm |
Zen |
Carl Zeiss Microscopy |
https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
|
|
| Software, algorithm |
Prism 6 |
GraphPad |
http://www.graphpad.com/scientific-software/prism/
|
|
