Appendix 1—table 1. Screen Summary.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Pathway | AdditionalInformation |
|---|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | UAS-EcR-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC03114}attP2/TM3, Sb1 | Bloomington Drosophila Stock Center | RRID:BDSC_50712 | Ecdysone | |
| Genetic reagent (D. melanogaster) | UAS-Ecr RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMJ22371}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_58286 | Ecdysone | |
| Genetic reagent (D. melanogaster) | UAS-btl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS02038}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_ 40871 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-btl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS02656}attP40 | Bloomington Drosophila Stock Center | RRID: BDSC_43544 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-btl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC04140}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_55870 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-btl-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS05005}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_ 60013 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-htl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01437}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_35024 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-htl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS04514}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_57313 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-htl-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMJ22375}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_ 58289 | FGFR | |
| Genetic reagent (D. melanogaster) | UAS-Galphaf-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL01545}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_43201 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphai-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01273}attP2/TM3, Sb1 | Bloomington Drosophila Stock Center | RRID:BDSC_34924 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphai-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00328}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_ 35407 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphai-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS02138}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_40890 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphao-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01129}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_34653 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphaq-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.JF02464}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33765 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphaq-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS03015}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_36775 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphaq-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL01048}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_36820 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-Galphas-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC03106}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_50704 | GPCR | |
| Genetic reagent (D. melanogaster) | UAS-ci: w[*]; P{w[ + mC] = UAS ci.HA.wt}3 | Bloomington Drosophila Stock Center | RRID:BDSC_32570 | Hedgehog | |
| Genetic reagent (D. melanogaster) | UAS-Ci-activated: w[*]; P{w[ + mC] = UAS HA.ci.m1-3*103}2 | Bloomington Drosophila Stock Center | RRID:BDSC_32571 | Hedgehog | |
| Genetic reagent (D. melanogaster) | UAS-ci-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC05801}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_64928 | Hedgehog | |
| Genetic reagent (D. melanogaster) | UAS-Hmgcr-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC03053}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_50652 | Hedgehog | |
| Genetic reagent (D. melanogaster) | UAS-hpo-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00006}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33614 | Hippo | |
| Genetic reagent (D. melanogaster) | UAS-hpo-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00046}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_35176 | Hippo | |
| Genetic reagent (D. melanogaster) | UAS-yki-activated: w[*]; P{y[ + t7.7] w[ + mC] = UAS yki.S111A.S168A.S250A.V5}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_28817 | Hippo | |
| Genetic reagent (D. melanogaster) | UAS-yki-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00041}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_34067 | Hippo | |
| Genetic reagent (D. melanogaster) | UAS-Akt: y1 w[1118]; P{w[ + mC] = UAS Akt.Exel}2 | Bloomington Drosophila Stock Center | RRID:BDSC_8191 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Akt-activated: w[*]; P{w[ + mC] = UAS-myr-Akt1.V}3/TM3, Sb1 | Bloomington Drosophila Stock Center | RRID:BDSC_50758 | InR | No longer available |
| Genetic reagent (D. melanogaster) | UAS-Akt-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00007}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33615 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Akt-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.JF02668}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_27518 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo: y1 w[*]†; P{w[ + mC] = UAS foxo.P}2 | Bloomington Drosophila Stock Center | RRID:BDSC_9575 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo: w[1118]; P{w[ + mC] = UASp foxo.S}3 | Bloomington Drosophila Stock Center | RRID:BDSC_42221 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo: w[1118]; P{w[ + mC] = UASp foxo.GFP}3 | Bloomington Drosophila Stock Center | RRID:BDSC_43633 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo: w[1118]; P{w[ + mC] = UASp foxo.GFP}2 | Bloomington Drosophila Stock Center | RRID:BDSC_44214 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo RNAi: y[1] sc[*] v[1] sev[21]; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00422}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_32427 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Foxo RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00793}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_32993 | InR | |
| Genetic reagent (D. melanogaster) | UAS-InR-DN: y1 w[1118]; P{w[ + mC] = UAS InR.K1409A}2 | Bloomington Drosophila Stock Center | RRID:BDSC_8252 | InR | |
| Genetic reagent (D. melanogaster) | UAS-InR-DN: y1 w[1118]; P{w[ + mC] = UAS InR.K1409A}3 | Bloomington Drosophila Stock Center | RRID:BDSC_8253 | InR | |
| Genetic reagent (D. melanogaster) | UAS-InR-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS03166}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_51518 | InR | |
| Genetic reagent (D. melanogaster) | UAS-Pi3K21B: y1 w[*]; P{w[ + mC] = UAS-Pi3K21B.HA}2 | Bloomington Drosophila Stock Center | RRID:BDSC_25899 | InR | |
| Genetic reagent (D. melanogaster) | UAS-dome-RNAi: w[1118]; P{GD14494}v36356 | Vienna Drosophila Resource Center | Stock #: 36356 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-dome-RNAi:P{KK104700}VIE-260B | Vienna Drosophila Resource Center | Stock #: 106071 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-hop-activated: w; UAS-hop[TumL]/CyO | PMID:7796812 | Jak-Stat | Dr. Norbert Perrimon(Harvard Medical School) | |
| Genetic reagent (D. melanogaster) | UAS-Stat92E-RNAi: w[1118]; P{GD4492}v43866 | Vienna Drosophila Resource Center | Stock #: 43866 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-Stat92E-RNAi:P{KK100519}VIE-260B | Vienna Drosophila Resource Center | Stock #: 106980 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-upd1 | PMID: 10346822 | Jak-Stat | Dr. David Strutt (Harvard Medical School) | |
| Genetic reagent (D. melanogaster) | UAS-upd1-RNAi: w[1118]; P{GD1158}v3282 | Stock #: 3282 | Jak-Stat | ||
| Genetic reagent (D. melanogaster) | UAS-upd1-RNAi: y[1] sc[*] v[1] sev[21]; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00545}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33680 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-upd2-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00901}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33949 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-upd2-RNAi:1sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00948}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33988 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-upd3-RNAi: y1 sc[ *]‡ v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00646}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_32859 | Jak-Stat | |
| Genetic reagent (D. melanogaster) | UAS-hep-activated: y1 w[1118]; P{w[ + mC] = UAS hep.CA}4 | Bloomington Drosophila Stock Center | RRID:BDSC_6406 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-hep-activated: w[*]; P{w[ + mC] = UAS Hep.Act}2 | Bloomington Drosophila Stock Center | RRID:BDSC_9306 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-kay: w[1118]; P{w[ + mC] = UAS-Fra}2 | Bloomington Drosophila Stock Center | RRID:BDSC_7213 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-kay-DN: w[1118]; P{w[ + mC] = UAS Fra.Fbz}5 | Bloomington Drosophila Stock Center | RRID:BDSC_7214 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-kay-DN: y1 w[1118]; P{w[ + mC] = UAS Fra.Fbz}7 | Bloomington Drosophila Stock Center | RRID:BDSC_7215 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-jra: y1 w[1118]; P{w[ + mC] = UAS-Jra}2 | Bloomington Drosophila Stock Center | RRID:BDSC_7216 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-kay-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00254}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33379 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-wgn-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC03962}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_55275 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-egr-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC03963}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_55276 | Jnk | |
| Genetic reagent (D. melanogaster) | UAS-Pvr-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01662}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_37520 | Pvr | |
| Genetic reagent (D. melanogaster) | UAS-Pvf1-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01958}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_39038 | Pvr | |
| Genetic reagent (D. melanogaster) | UAS-Pvr-DN: w[1118]; P{w[ + mC] = UASp Pvr.DN}D1/CyO | Bloomington Drosophila Stock Center | RRID:BDSC_58430 | Pvr | |
| Genetic reagent (D. melanogaster) | UAS-Pvr-DN: w[1118]; P{w[ + mC] = UASp Pvr.DN}D7 | Bloomington Drosophila Stock Center | RRID:BDSC_58431 | Pvr | |
| Genetic reagent (D. melanogaster) | UAS-aop: w[*]; P{w[ + mC] = UAS aop.WT}Ia/CyO | Bloomington Drosophila Stock Center | RRID:BDSC_5790 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-aop-activated: w[*]; P{w[ + mC] = UAS aop.ACT}IIa | Bloomington Drosophila Stock Center | RRID:BDSC_5789 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-pnt-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01452}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_35038 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-rl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00173}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_34855 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-rl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00215}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_36058 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-sev-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.JF02393}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_36778 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-sev-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMC04136}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_55866 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-tor-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00021}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_33627 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-tor-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP.HMJ22419}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_58312 | RTK | |
| Genetic reagent (D. melanogaster) | UAS-Mad:P{ry[ + t7.2] = hsFLP}12, y1 w[*]; P{w[ + mC] = UAS Mad.FLAG}2; P{y[ + t7.7] w[ + mC] = mir-ban-lacZ.brC12}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_44256 | TGFβ | |
| Genetic reagent (D. melanogaster) | UAS-Mad-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.GLV21013}attP2/TM3, Sb1 | Bloomington Drosophila Stock Center | RRID:BDSC_35648 | TGFβ | |
| Genetic reagent (D. melanogaster) | UAS-Mad-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL01527}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_43183 | TGFβ | |
| Genetic reagent (D. melanogaster) | UAS-Smox-RNAi:1sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS02203}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_41670 | TGFβ | |
| Genetic reagent (D. melanogaster) | UAS-Smox-RNAi: y1 v1; P{y[ + t7.7] v[ + t1.8] = TRiP .GL01476}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_43138 | TGFβ | |
| Genetic reagent (D. melanogaster) | UAS-cact-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00084}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_34775 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-cact-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00627}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_37484 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-dl: y1 w[*]; P{w[ + mC] = UAS dl.H}2 | Bloomington Drosophila Stock Center | RRID:BDSC_9319 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-dl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00727}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_32934 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-dl-RNAi:y1sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS00028}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_34938 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-dl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00610}attP40 | Bloomington Drosophila Stock Center | RRID:BDSC_36650 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-dl-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP .GL00676}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_38905 | Toll | |
| Genetic reagent (D. melanogaster) | UAS-arm-RNAi: y1 sc[*] v1 sev21; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS01414}attP2 | Bloomington Drosophila Stock Center | RRID:BDSC_35004 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-pan: y1 w[1118]; P{w[ + mC] = UAS pan.dTCF}24/CyO | Bloomington Drosophila Stock Center | RRID:BDSC_4837 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-pan: y1 w[1118]; P{w[ + mC] = UAS pan.dTCF}4 | Bloomington Drosophila Stock Center | RRID:BDSC_4838 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-pan-constitutive repressor: y[1] w[1118]; P{w[ + mC] = UAS pan.dTCFDeltaN}4 | Bloomington Drosophila Stock Center | RRID:BDSC_4784 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-pan-constitutive repressor: y[1] w[1118]; P{w[ + mC] = UAS pan.dTCFDeltaN}5 | Bloomington Drosophila Stock Center | RRID:BDSC_4785 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-pan-RNAi: y v; P{y[ + t7.7] v[ + t1.8] = TRiP.HMS02015}attP40/CyO | Bloomington Drosophila Stock Center | RRID:BDSC_40848 | Wnt | |
| Genetic reagent (D. melanogaster) | UAS-wg-RNAi: w[1118]; P{GD5007}v13352 | Vienna Drosophila Resource Center | Stock #: 13352 | Wnt |
*
n = at least 20–199 testes for all lines.
†
lines listed in the key resource table are not repeated here.
‡
no lines listed here had significant numbers of dividing hub cells compared to UAS-GFP-RNAi controls.