Table 3. Hub cell fate conversion after EGFR pathway activation.
Gal4 driver | UAS lines (BDSC #) | Days at 29 °C | % Testes with GFP-marked cells outside the hub |
---|---|---|---|
E132-Gal4, Gal80ts | UAS-G-TRACE (28280) | 8 | 0% (n = 0/39) |
UAS-G-TRACE (28280); UAS-Egfr Type II (9533) | 61% (n = 19/31)**** | ||
UAS-G-TRACE (28280); UAS-Pointed.P1 (869) | 38% (n = 19/50)**** | ||
UAS-G-TRACE (28280); UAS-Pointed.P2 (399) | 22% (n = 4/18)** | ||
UAS-G-TRACE (28280); UAS-Sprouty-RNAi (36709) | 36% (n = 32/89)**** | ||
UAS-G-TRACE (28280); UAS- Pten-RNAi (33643) | 18% (n = 6/34)** | ||
E132-Gal4, Gal80ts | UAS-G-TRACE (28281) | 8 | 0% (n = 0/84) |
UAS-Egfr Type I (9534); UAS-G-TRACE (28281) | 2% (n = 1/47)ns |
Percentages in bold are significant compared to the negative control (corresponding UAS-G-TRACE alone).
Fisher’s Exact Test: **p < 0.01, ****p < 0.0001, ns = not significant.