Table 4. Overexpression of EGF ligands in the adult testis niche does not cause hub cell proliferation.
Gal4 driver | UAS line | Source | Days at 31 °C | % Testes with PH3-positive hub cells |
---|---|---|---|---|
C587-Gal4, Gal80ts | UAS-GFP-RNAi | BDSC 9330 or 9331 | 7 | 0% (n = 0/294)* |
UAS-secreted spitz | BDSC 63134 | 7–9 | 0% (n = 0/219)a,ns | |
UAS-secreted spitz | BDSC 58436 | 7 | 0% (n = 0/75)a,ns | |
UAS-gurken ΔTC | Queenan et al., 1999 | 7 | 0% (n = 0/82)a,ns | |
UAS-secreted gurken | BDSC 58417 | 7 | 0% (n = 0/118)a,ns | |
UAS-secreted keren | Urban et al., 2002 | 7 | 0% (n = 0/113)a,ns | |
UAS-vein | Schnepp et al., 1996 | 6 | 0% (n = 0/96)a,ns | |
E132-Gal4, Gal80ts | UAS-GFP-RNAi | BDSC 9330 or 9331 | 7 | 0% (n = 0/237)* |
UAS-secreted spitz | BDSC 63134 | 7 | 0% (n = 0/208)a,ns | |
UAS-secreted spitz | BDSC 58436 | 7 | 0% (n = 0/92)a,ns | |
UAS-gurken ΔTC | Queenan et al., 1999 | 7 | 0% (n = 0/86)a,ns | |
UAS-secreted gurken | BDSC 58417 | 7 | 1% (n = 1/77)a,ns | |
UAS-secreted keren | Urban et al., 2002 | 7 | 0% (n = 0/113)a,ns | |
UAS-vein | Schnepp et al., 1996 | --- | --- | |
C587-Gal4, Gal80ts | UAS-secreted spitz +UAS-Argos-RNAi | BDSC 63134 +BDSC 28383 | 7–9 | 2% (n = 4/203)b,ns |