| Antibody |
Anti-Human BDCA1 (mouse monoclonal) |
Biolegend |
Cat# 354,208 |
Clone: 201 A (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD117 (mouse monoclonal) |
Biolegend |
Cat# 313,206 |
Clone: 104D2 (APC) (1:200 dilution) |
| Antibody |
Anti-Human CD11c (mouse monoclonal) |
Biolegend |
Cat# 301,604 |
Clone: 3.9 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD123 (mouse monoclonal) |
Biolegend |
Cat# 306,014 |
Clone: 6H6 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD127 (mouse monoclonal) |
Biolegend |
Cat# 351,320 |
Clone: A019D5 (PE/Cyanine7) (1:200 dilution) |
| Antibody |
Anti-Human CD14 (mouse monoclonal) |
Biolegend |
Cat# 325,604 |
Clone: HCD14 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD16 (mouse monoclonal) |
Biolegend |
Cat# 980,104 |
Clone: 3G8 (APC) (1:400 dilution) |
| Antibody |
Anti-Human CD19 (mouse monoclonal) |
Biolegend |
Cat# 302,206 |
Clone: HIB19 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD1a (mouse monoclonal) |
Biolegend |
Cat# 300,104 |
Clone: HI149 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD20 (mouse monoclonal) |
Biolegend |
Cat# 302,304 |
Clone: 2H7a (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD22 (mouse monoclonal) |
Biolegend |
Cat# 363,508 |
Clone: S-HCL-1 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD3 (mouse monoclonal) |
Biolegend |
Cat# 317,306 |
Clone: OKT3 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD34 (mouse monoclonal) |
Biolegend |
Cat# 343,504 |
Clone: 581 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD4 (mouse monoclonal) |
Biolegend |
Cat# 317,428 |
Clone: OKT4 (PerCP/Cyanine5.5) (1:200 dilution) |
| Antibody |
Anti-Human CD4 (mouse monoclonal) |
Biolegend |
Cat# 317,408 |
Clone: OKT4 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human CD45 (mouse monoclonal) |
BD |
Cat# 560,178 |
Clone: 2D1 (APC/H7) (1:200 dilution) |
| Antibody |
Anti-Human CD56 (mouse monoclonal) |
Biolegend |
Cat# 318,306 |
Clone: HCD56 (PE) (1:200 dilution) |
| Antibody |
Anti-Human CD8 (mouse monoclonal) |
Biolegend |
Cat# 300,924 |
Clone: HIT8a (PerCP/Cyanine 5.5) (1:200 dilution) |
| Antibody |
Anti-Human CRTH2 (rat monoclonal) |
Biolegend |
Cat# 350,116 |
Clone: BM16 (PerCP/Cyanine5.5) (1:200 dilution) |
| Antibody |
Anti-Human FcεR1α (mouse monoclonal) |
Biolegend |
Cat# 334,608 |
Clone: AER-37 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human TBX21 (mouse monoclonal) |
ebioscience |
Cat# 25-5825-82 |
Clone: ebio4B10 (PE/Cyanine7) (1:200 dilution) |
| Antibody |
Anti-Human TCRα/β (mouse monoclonal) |
Biolegend |
Cat# 306,706 |
Clone: IP26 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human TCRγ/δ (mouse monoclonal) |
Biolegend |
Cat# 331,208 |
Clone: B1 (FITC) (1:200 dilution) |
| Antibody |
Anti-Human TCF7 (rabbit monoclonal) |
Cell Signaling |
Cat# 37,636 s |
Clone: C63D9 (APC) (1:200 dilution) |
| Antibody |
Anti-Human IL-13 (rat monoclonal) |
Biolegend |
Cat# 501,908 |
Clone: JES10-5A2 (APC) (1:200 dilution) |
| Antibody |
Anti-Human AREG (mouse monoclonal) |
ebioscience |
Cat# 17-5370-42 |
Clone: AREG559 |
| Antibody |
mouse IgG1, k isotype control (mouse monoclonal) |
Biolegend |
Cat# 400,112 |
Clone: MOPC-21 (PE) (1:200 dilution) |
| Antibody |
mouse IgG1, k isotype control (mouse monoclonal) |
Biolegend |
Cat# 400,120 |
Clone: MOPC-21 (APC) (1:200 dilution) |
| Antibody |
Rabbit IgG, isotype control (rabbit monoclonal) |
Cell Signaling |
Cat# 3,452 S |
(Alexa Fluor 647) (1:200 dilution) |
| Antibody |
Rat IgG1, k isotype control (rat monoclonal) |
Biolegend |
Cat# 400,412 |
Clone: RTK2071 (APC) (1:200 dilution) |
| Biological Samples (Homo sapiens) |
PBMCs |
New York Biologics |
https://www.newyorkbiologics.com/
|
|
| Biological Samples (Homo sapiens) |
PBMCs |
MassCPR |
https://masscpr.hms.harvard.edu/
|
|
| Biological Samples (Homo sapiens) |
PBMCs |
MGH Pediatric COVID-19 Biorepository |
|
|
| Commercial assay or kit |
Cell stimulation cocktail |
eBioscience |
Cat# 00-4970-03 |
|
| Chemical compound, drug |
Protein transport inhibitor |
eBioscience |
Cat# 00-4980-03 |
|
| Chemical compound, drug |
TRIzol reagent |
Invitrogen |
Cat# 15596018 |
|
| Commercial assay or kit |
AMPure XP beads |
Beckman Culter |
Cat# A63880 |
|
| Commercial assay or kit |
ExoSAP-IT |
Affymetrix |
Cat# 78,200 |
|
| Commercial assay or kit |
Live and Dead violet viability kit |
Invitrogen |
Cat# L-34963 |
|
| Commercial assay or kit |
Foxp3 /Transcription Factor Staining Buffer Set |
eBioscience |
Cat# 00-5523-00 |
|
| Commercial assay or kit |
HiScribe T7 High Yield RNA Synthesis Kit |
NEB |
Cat# E2040S |
|
| Commercial assay or kit |
NEBNext Ultra II Non directional Second Strand Synthesis Module |
NEB |
Cat# E6111L |
|
| Software, algorithm |
R computer software environment (version 4.0.2) |
The R Foundation R Development Core Team, 2020
|
https://www.r-project.org/
|
|
| Software, algorithm |
FlowJo |
FlowJo, LLC |
https://www.flowjo.com/
|
|
| Software, algorithm |
tidyverse v1.3.1 |
Wickham et al., 2019
|
https://www.tidyverse.org
|
|
| Software, algorithm |
ggplot2 v3.3.3 |
Wickham, 2016
|
https://ggplot2.tidyverse.org
|
|
| Software, algorithm |
ggpubr v0.4.0 |
Kassambara, 2020
|
https://rpkgs.datanovia.com/ggpubr/
|
|
| Software, algorithm |
ComplexHeatmap v2.4.3 |
Gu et al., 2016
|
https://github.com/jokergoo/ComplexHeatmap
|
|
| Software, algorithm |
emmeans v1.6.0 |
Wieduwilt et al., 2020
|
https://CRAN.R-project.org/package=emmeans
|
|
| Software, algorithm |
lme4 v1.1–27 |
Bates et al., 2015
|
https://cran.r-project.org/web/packages/lme4/index.html
|
|
| Software, algorithm |
lmerTest v3.1–3 |
Kuznetsova et al., 2017
|
https://cran.r-project.org/web/packages/lmerTest/index.html
|
|
| Software, algorithm |
DolphinNext RNA-Seq pipeline (Revision 4) |
Yukselen et al., 2020
|
https://github.com/UMMS-Biocore/dolphinnext
|
|
| Software, algorithm |
STAR v2.1.6 |
Dobin et al., 2013
|
https://github.com/alexdobin/STAR
|
|
| Software, algorithm |
RSEM v1.3.1 |
Li and Dewey, 2011
|
http://deweylab.github.io/RSEM/
|
|
| Software, algorithm |
DESeq2 v1.28.1 |
Love et al., 2014
|
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
|
| Software, algorithm |
clusterProfiler v3.16.1 |
Yu et al., 2012
|
https://guangchuangyu.github.io/software/clusterProfiler/
|
|
