Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 11;10:832431. doi: 10.3389/fchem.2022.832431

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Ewert, Günther, Miglioli, Falke, Reinke, Niebling, Günther, Han, Srinivasan, Brognaro, Lieske, Lorenzen, Garcia-Alai, Betzel, Carcelli, Hinrichs, Rogolino and Meents.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The bound compounds interfere with key residues for substrate binding at the S1 (yellow frame) and S2 (pink frame) site. (A) Overlay of ubiquitin bound to PLpro (PDB: 6xaa, PLpro light green, ubiquitin green) and the hydrazone bound structure (PLpro grey, H1 magenta) showing the rearrangement of several PLpro-ubiquitin interacting side chains to form hydrogen bonds with the compound. (B,C) Overlay of PLpro complexed with human ISG15 (PDB: 7rbs, PLpro light pink, ISG15 purple) and PLpro bound with hydrazone H1 and thiosemicarbazone T2 (PLpro grey, H1 magenta, T2 green) at the S1 and S2 sites respectively highlighting the structural differences in binding the compound or substrate.