Figure 3.

HUA promotes macrophage-derived foam cell formation in THP-1 and RAW264.7 cells. THP-1 and RAW264.7 cells were treated with oxLDL (100 μg/ml) or coincubated with UA (15 mg/dl) and with or without Fer-1 (2 μM) for 24 h. (a and b) Oil red O staining to evaluate the effect of HUA and Fer-1 on the formation of foam cells. (c and d) Quantification of the lipid accumulation in THP-1 and RAW264.7 cells. Data are means ± SD, n = 3. Fer-1: ferrostatin-1; HUA: high level of uric acid; oxLDL: oxidized low-density lipoprotein; UA: uric acid. ^∗∗∗P < 0.001.