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. 2022 Feb 10;15(3):428–442. doi: 10.1038/s41385-021-00478-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Expression of CD45RA and CCR7 by CD8 T cells isolated from the indicated tissues of naïve Babraham pigs (n = 3). Quadrants show the proportion of each population. b Mean frequency (± SD) of TDE (CD45RA+, CCR7–), naïve (CD45RA + , CCR7 + ), TCM (CD45RA-, CCR7 + ) and TEM (CD45RA-, CCR7-) in CD8 T cells. c CD8 + T cells from PBMC of naïve Babraham pigs (n = 3) were sorted according to their expression of CD45RA and CCR7. The sorted cells were stimulated with PMA and Ionomycin for 0, 2, 4 and 6 h and TNF and IFNγ secretion measured by intracellular cytokine staining. Each symbol represents one animal, this experiment was repeated twice. d Representative FACS plot showing the secretion of IFNγ and TNF after 6 h stimulation in TDE (white panel), naïve (blue panel), TCM (grey panel) and TEM (red). Mean proportion of IFNγ single (top left), double producer (top right) and single TNF + (bottom right) T cells are reported. e CD69 expression in differentiated effector (TDE, white), naïve (blue), central memory (TCM, grey) and effector memory (TEM, red) in CD8 T cells in the tissues analyzed.