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. 2022 Feb 10;15(3):428–442. doi: 10.1038/s41385-021-00478-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Sorting strategy for the isolation of DFE + T cells for RNA-seq. b Venn diagram shows the number of significant differentially expressed genes (padj value<0.05, and |log2 fold change | > 1) between 7DPI versus (vs) 21 DPI, 21DPI vs 63DPI and 63DPI vs 7DPI samples (n = 3). c Volcano plot showing upregulated genes in 21DPI vs 7DPI and 63DPI vs 21DPI comparison. d Heatmap of selected genes from KEGG Pathway analysis related to cytokine production, T cell differentiation and TGFβ pathway (enrichment score of 0.76, 0.88 and 0.83 respectively).