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. 2022 Feb 10;15(3):428–442. doi: 10.1038/s41385-021-00478-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — (a) Lymphocytes isolated at different time points from blood, TBLN and BAL were stimulated with H1N1pdm09 MOI = 1. Following 18 h of stimulation, cells were labelled using tetramers and cytokines quantified using intracellular cytokine staining. Representative plots of AAV + (on left) and DFE + (right) T cells secreting IFNγ, TNF and IL-2 cytokines, from lymphocytes isolated 14 DPI. b Mean frequency (±SEM) of tetramer+ cells secreting IFNγ, TNF and IL-2 from PBMC (top panels), TBLN (in the middle) and BAL (bottom). The right Y axes shows the mean frequency of AAV + (in blue, left panels) and DFE + (in black, right panels) within CD8 T cells. Data shown are mean of 3/4 individuals per timepoint. Two-way ANOVA was used for comparison of each cytokine population between DFE+ and AAV+ cells.