Fig. 5. Pyroptosis upon Gpr35 deletion is caspase-11 dependent.

a Protein expression of cleaved GSDMD, cleaved caspase-11 and cleaved caspase-1 in crypt samples isolated from the proximal colon obtained from Gpr35^f/fVil⁺ (n = 3) and Gpr35^wt co-housed littermates (n = 3). b Densitometry analysis of (a). c Protein expression of cleaved GSDMD and cleaved caspase-11 in sorted goblet cells and colonocytes obtained from Gpr35^f/fVil⁺ (n = 3) and Gpr35^wt co-housed littermates (n = 3). d Densitometry analysis of (c). e Flow cytometry assessment of cell apoptosis (TUNEL) percentage in goblet cells obtained from the proximal colon of Gpr35^f/fVil⁺ (red histogram) and Gpr35^wt (gray histogram) co-housed littermates. Numbers in histograms indicate the percentage of apoptotic goblet cells. The blue histogram represents the negative control of the assay. f Protein expression of cleaved GSDMD and cleaved caspase-11 in proximal colon explant obtained from Gpr35^wt mice treated with ML194 at 10 μM for 3 h. g Densitometry analysis of (f). h Protein expression of cleaved GSDMD and cleaved caspase-11 in proximal colon explants obtained from Gpr35^wt mice treated with either Zaprinast or LPA at 10 μM for 1.5 h followed by OMVs treatment at 10 μg for 1.5 h. i Densitometry analysis of (h). Each dot represents one animal with medians. Data are represented as mean ± SEM *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 by Mann–Whitney test.