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. 2022 Feb 15;15(3):504–514. doi: 10.1038/s41385-022-00484-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c Eo-Cre x Tgfb^fl/fl mice and their wild-type littermates were infected with C. rodentium for 12 days. Absolute counts per organ of all colonic lamina propria CD4⁺ T-cells and CD4⁺ Foxp3⁺ Tregs are shown in a, and absolute counts per organ of colonic Foxp3⁺ Nrp-1⁻ Tregs and of RORγt⁺ Nrp-1⁻ Tregs are shown in b and c relative to uninfected age-matched controls. Data in a–c are pooled from two independent studies. d–f Eo-Cre x Tgfb^fl/fl mice and their wild-type littermates were infected with C. rodentium for 12 days, and additionally injected with CD45.1⁺ OT-II splenocytes and one intravenous dose of OVA peptide 7 days prior to the termination of the study. Absolute counts per organ of the indicated adoptively transferred CD45.1⁺ CD4⁺ T-cell and Treg populations are shown in d–f. Data in d–f are pooled from two independent studies. g–k Eo-Cre x Tgfb^fl/fl mice and their wild-type littermates were intraperitoneally sensitized twice with alum-adjuvanted ovalbumin at 5 and 7 weeks of age and challenged on four consecutive days with orally administered ovalbumin 4 weeks after the first sensitization (s/c). Control mice were mock-sensitized and -challenged with PBS only. TGF-β production by colonic eosinophils as determined by flow cytometry is shown in g; frequencies of CD4⁺ T-cells among all colonic CD45⁺ cells and of Foxp3⁺ Tregs among all CD4⁺ T-cells are shown in h. The frequencies of colonic Foxp3⁺ Nrp-1⁻ Tregs and of RORγt⁺ Nrp-1⁻ Tregs among CD4⁺ T-cells are shown in i. Cumulative anaphylaxis score over 3 days are shown in j, and OVA-specific IgE in serum, and IL-13 and IL-5 production by OVA-restimulated splenocytes, as assessed by ELISA, is shown in k. Data from two independent studies are pooled in g–k. Statistical comparisons were performed by Kruskal–Wallis test followed by Dunn’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant.