Figure 1. Analysis of absolute numbers and frequencies of TREG in kidney transplant recipients.
(a) Representative example of the gating strategy used for the identification of TREG (CD3+CD4+CD25hiCD127−FoxP3+ cells) in blood by flow cytometry. (b) Representative examples of flow cytometry analysis, and dot plots of percentages and cell counts per million PBMC of TREG cells are displayed; HC (N=18), DSA− (N=48), DSA+ABMR− (N=28) and DSA+ABMR+ (N=20) patients. Kruskal-Wallis with Dunn’s post-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Each dot represents one subject and horizontal lines are mean values.