Skip to main content
. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Kidney Int. 2022 Jan 26;101(5):1003–1016. doi: 10.1016/j.kint.2021.12.027

Figure 2. High dimensional flow cytometry analyses of TREG in kidney transplant recipients.

Figure 2.

(a) t-SNE projections were generated using a concatenated file of N= 73,600 TREG cells from HC (N=5), DSA− (N=20), DSA+ABMR− (N=20) and DSA+ABMR+ (N=20) patients; panels display expression levels of indicated markers (MFI). (b) t-SNE projections of TREG densities in the four groups using N=18,400 cells from each group, shown in panel a. (c) t-SNE map overlaid with 12 TREG clusters delineated by SPADE clustering of the concatenated file, as in panel a. (d) Heatmap showing the expression of markers for each TREG cluster according to transformed MFI ratio. * indicates the TREG clusters significantly diminished in frequencies in the DSA+ABMR+ group. (e) Stacked bar plot showing TREG cluster distribution based on SPADE clustering as in panel c. Clusters 1, 3, 6, 10 and 11 are significantly different in their proportions across the indicated groups. Kruskal-Wallis with Dunn’s post-test. (f) Representative examples of flow cytometry analysis and dot plot of percentages of T-bet+ CXCR5+ cells in TREG are displayed; HC (N=18), DSA− (N=48), DSA+ABMR− (N=28) and DSA+ABMR+ (N=20) patients. Kruskal-Wallis with Dunn’s post-test. *P < 0.05; **P < 0.01; ****P < 0.0001. Each dot represents one subject and horizontal lines are mean values.