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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: J Invest Dermatol. 2021 Oct 28;142(5):1280–1290.e7. doi: 10.1016/j.jid.2021.10.009

Figure 1. Determination of Presence or Absence of Human Papillomavirus for Vulvar Squamous Cell Carcinoma.

Figure 1.

a. Representative images of RNA HPV-ISH results separated by score. Bar = 50 μm

b. Expression scores for HPV-ISH for VSCC and control samples.

c. Representative image of HPV PCR. Positive control represents 10 pg of the HPV-16 added to the outer PCR reaction. Negative controls used include water and a normal vulvar tissue sample without history of HPV infection or neoplasm.

d. PCR and HPV-ISH comparison results.

e. Kaplan–Meier curves for overall survival and progression-free survival by HPV-final consensus status and compared by log-rank test with a p-value <0.05 considered significant.