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. Author manuscript; available in PMC: 2023 Apr 26.
Published in final edited form as: Circulation. 2022 Jan 21;145(17):1339–1355. doi: 10.1161/CIRCULATIONAHA.121.057641

Fig. 1. 4F overexpression induces cell cycle reprogramming in hiPS-CMs.

Fig. 1.

(a) Principal component analysis (PCA) of RNA-seq data from mature hiPS-CMs (60 day old) infected with either LacZ (control) or 4F for 24, 48, 72 or 144 h (n = 3). (b) Volcano plot demonstrating the number of genes that are significantly upregulated or downregulated 48 h post-4F adenovirus overexpression compared to the LacZ group (padj<0.05). (c) The bar graph shows the top GO terms for the significantly upregulated genes from RNA-seq data comparing hiPS-CMs infected with either LacZ or 4F for 48 h (padj<0.05). The GO terms reflect mostly upregulation of cell cycle genes. (d) Row normalized Z score heatmap shows a temporal expression of over 200 genes related to the cell cycle (n=3 in each sample). Source data 1 lists FPKM values for all RNAseq data and for the heart maps. Source data 2 contains the full list of the GO terms and the genes included in each GO term.