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. Author manuscript; available in PMC: 2023 May 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2022 Feb 24;61(19):e202116802. doi: 10.1002/anie.202116802

Figure 3.

Figure 3.

Cell labeling with the nanoprobe and PAPSI in the cells. a) Fluorescence images of the HeLa cells incubated with the nanoprobe (0.125 nM) for 2, 6, or 10 h at 37 °C. Scale bar: 20 μm. b, c) Number of nanoprobes taken up by the cells determined by ICP-MS at b) different incubation time (2–10 h) with the same probe concentration (0.125 nM) or c) different probe concentrations (0.0625–2 nM) with the same incubation time (6 h). Results are presented as Mean ± SD (three wells per group in 6-well cell culture plates). d-g) PAPSI images of labeled HeLa cells (2 × 104 NPs per cell) in 10 μL PBS (pH 7.4) in a transparent plastic tube at indicated cell number. d): 106; e): 105, f): 2 × 104; g): 5 × 103 with indicated PAPSI cycle number. Scale bars: 2 mm.