a - c) Representative western blots and densitometry analysis in kidney tissue lysates for the expression of fibronectin (FN), TGF-β1, alpha-smooth muscle actin (α-SMA), CD206, arginase-I (Arg-I), and galectin-3 (Gal-3) from mice fed with control (Ctl) or adenine diet (AD) for 28 days in a)
Mfn1fl/fl,LysM-Cre-−/− and Mfn1fl/fl,LysM-Cre+/− mice; b)
Mfn2fl/fl,LysM-Cre-−/− and Mfn2fl/fl,LysM-Cre+/− mice, and c) Wild-type and Mfn1/Mfn2 double knockout (DKO) mice, normalized to GAPDH, n = 6 per group. Data are mean ± SEM representative of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, analyzed by one-way ANOVA followed by Newman-Keuls post-hoc test.