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. 2022 Apr 25;13(4):404. doi: 10.1038/s41419-022-04852-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — HBV cccDNA was extracted at day 5 post HBV infection using the Hirt method. Real-time PCR analysis was performed using cccDNA-specific primers upon shRNA knockdown (A) or NU7026 treatment (D). Droplet digital PCR (ddPCR) was also carried out to detect the numbers of cccDNA after PRKDC knockdown (B) or inhibitor treatment (E). The decrease in total HBV RNAs or pgRNA expression was calculated as the ratio of normalized HBV RNAs or pgRNA to cccDNA after PRKDC knockdown (C) or inhibitor treatment (F). **P < 0.01; n.s. not significant.