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. 2022 Apr 25;6:28. doi: 10.1038/s41698-022-00270-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Correlation plot of qRT-PCR data compared to RNA-sequencing data. P value was calculated by t-test. b, c qRT-PCR Ct values of top four LVQ genes identified from MM versus NC (b) and top 5 LVQ genes identified from HCC versus NC (c). Center-line for boxplots in both b and c indicates the median value across all patients in that group, and the hinges represent the lower (Q1) and upper (Q3) quartile, with whiskers extending to the minimum and maximum of the resulting distribution.