Fig. 1. Comparison of the WT and itrA::ISAba13 strains.
a Sequence alignment of the K-loci of the WT and itrA::ISAba13 mutant. ISAba13 (represented in yellow) interupts itrA gene. IRR/IRL: respectively, inverted repeat right and left, tnpA: transposase gene, DTR: Direct Target Repeats of 9 nucleotides flanking ISAba13. Dark gray color indicates 100% sequence identity. b TEM of the WT and itrA::ISAba13 strains. Scale bar: 500 nm. c Macrocolonies and density gradients. Scale bar: 0.5 cm. d Survival of G. mellonella. Y axis: survival of larvae (%), x axis: time post-inoculation (days), 10 larvae were infected for each condition and experiments were performed in biological duplicates.