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. 2021 Dec 23;16(5):1473–1477. doi: 10.1038/s41396-021-01179-3

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Fig. 2 — a Opaque colonies isolated by plating itrA::ISAba13 bacteria harvested near the inhibition zone of a Colistin Etest. Bacteria near the inhibition zone (red square on left panel) were collected with a plastic loop and re-isolated on solid medium (right panel). The non-reversible control ∆itrA strain shows 100% of translucent colonies. Scale bar: 0.2 cm. b Sequence alignment of the K-loci of the itrA::ISAba13 natural mutant strain and Revertant CO and PO-B. c Macrocolonies phenotype on solid media and density gradients. Scale bar: 0.5 cm. d Survival of G. mellonella over time. Y axis: survival of larvae (%), x axis: time post-inoculation (days). 10 larvae were infected for each condition and experiments were performed in biological duplicates.