For 50 mL T cell culture medium, the recipe is listed as follow:

Reagent	Final concentration	Amount
X-VIVOTM15	1×	45 mL
Inactivated fetal bovine serum	10%	5 mL
IL-2 (30,000 U/mL)	30 U/mL	50 μL
IL-7 (5 mg/mL)	5 ng/mL	50 μL
IL-15 (5 mg/mL)	5 ng/mL	50 μL
Penicillin-Streptomycin solution	1×	500 μL
Total	n/a	50 mL

Store at 4°C for one week, do not prepare too much because the IL-2 is easy to degrade in the culture medium.