For 50 mL AAV293 cell culture medium, the recipe is listed as follow:

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle’s Medium	1×	45 mL
Fetal Bovine Serum	10%	5 mL
Penicillin-Streptomycin solution	1×	500 μL
Total	n/a	50 mL

Store at 4°C for one month.