For 1 L 2YT medium, the recipe is listed as follow:
| Reagent | Final concentration | Amount |
|---|---|---|
| Tryptone | 16 g/L | 16 g |
| Yeast extract | 12 g/L | 12 g |
| NaCl | 5 g/L | 5 g |
| Total | n/a | 1 L |
Store at room temperature for one month after autoclaving.
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