For 50 mL T cell isolation buffer, the recipe is listed as follow:

Reagent	Final concentration	Amount
PBS without Ca2+ and Mg2+	1×	49 mL
BSA (10%, w/v)	0.1%	500 μL
EDTA (200 mM)	2 mM	500 μL
Total	n/a	50 mL

Store at 4°C for one month.