Volume of original stock or previous dilution (μL) | Volume of nuclease free water (μL) | Molecules per μL |
---|---|---|
10 | 90 | 2 × 109 |
10 of 2 × 109 dilution | 90 | 2 × 108 |
10 of 2 × 108 dilution | 90 | 2 × 107 |
10 of 2 × 107 dilution | 90 | 2 × 106 |
10 of 2 × 106 dilution | 90 | 2 × 105 |
10 of 2 × 105 dilution | 90 | 2 × 104 |