| Dilution series | Volume of sample (μL) | Volume of nuclease free water (μL) | Total dilution factor |
|---|---|---|---|
| Dilution 1 | 5 μL stock | 45 | 10× |
| Dilution 2 | 5 μL Dilution 1 | 95 | 200× |
| Dilution 3 | 20 μL Dilution 2 | 80 | 1,000× |
| Dilution 4 | 20 μL Dilution 3 | 80 | 5,000× |
| Dilution 5 | 20 μL Dilution 4 | 80 | 25,000× |
| Dilution 6 | 20 μL Dilution 5 | 80 | 125,000× |
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