Material	Safety considerations
HSV H129 and PRV-Bartha	Follow appropriate local Biosafety Level-2 institutional protocols. We suggest personal protective equipment of at least consisting of two layers of nitrile gloves, mask, eye protection, and disposable gown. We prefer to disinfect tools and areas with 70% alcohol or bleach immediately after use followed by sterilization in an autoclave when possible.
Paraformaldehyde/Formalin (PFA)	Eye protection, nitrile gloves, work under snorkel or fume hood
Methanol	Eye protection, nitrile gloves
Hydrogen Peroxide	Eye protection, nitrile gloves
Triton X-100	Eye protection, nitrile gloves
Dimethyl sulfoxide (DMSO)	Eye protection, nitrile gloves, work under snorkel or fume hood
Donkey Serum	Eye protection, nitrile gloves
Antibodies	Eye protection, nitrile gloves
Tween-20	Eye protection, nitrile gloves
Dichloromethane (DCM)	Eye protection, double nitrile gloves, work under snorkel or fume hood. Remove gloves immediately if dichloromethane gets on gloves.
Dibenzyl ether/benzyl ether (DBE)	Eye protection, nitrile gloves. Dibenzyl ether can be removed from surfaces using 70% ethanol.