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The reproducibility of the serial passage experiments was assessed by repeating the experiment sequentially with 15 clinical isolates of E. coli incubated with ciprofloxacin (Cipro)(●), levofloxacin (Levo)(■), and trovafloxacin (Trova)(▴). The 15 isolates were serially passaged in two consecutive experiments, and the results were compared to each other and to those for the earlier initial study with 20 different clinical isolates of E. coli.
