Table 2.
Summary of Symbiodiniaceae-infecting dinoflagellate RNA virus (“dinoRNAV”) “aminotypes” (unique amino acid sequences) specific to the heat or control treatment based on amplicon sequencing.
| Aminotype ID | N_Observations | Colony | Time observed (h) | Mean relative abundance (%) ± SD | Closest match |
|---|---|---|---|---|---|
| Heat treatment | |||||
| 22 | 3 | 4 | 12, 24, 72 | 0.05 ± 0.06 | Plutea_AminoType17 |
| 25 | 2 | 3 | 24, 72 | 1.70 | Plutea_AminoType21 |
| 37 | 1 | 2 | 4 | 0.10 | Plutea_AminoType21 |
| 44 | 2 | 1 | 4, 12 | 0.01 | Plutea_AminoType13 |
| 68 | 4 | 2,3 | 4 (2,3); 24, 72 (3) | 0.02 ± 0.02 | Plutea_AminoType21 |
| 72 | 1 | 3 | 4 | 0.02 | Pdamicornis_AminoType1 |
| 76 | 5 | 1,2,5 | 4, 24 (2); 12 (1, 2); 72 (5) | 0.03 ± 0.05 | Plutea_AminoType13 |
| 80 | 1 | 2 | 108 | 0.03 | Plutea_AminoType21 |
| 81 | 1 | 4 | 108 | 0.03 | Plutea_AminoType5 |
| 87 | 1 | 4 | 24 | 0.02 | Plutea_AminoType10 |
| 104 | 2 | 3 | 24, 72 | 0.16 | Plutea_AminoType21 |
| 107 | 3 | 4 | 24, 72, 108 | 0.04 ± 0.02 | Gfascicularis_AminoType1 |
| 112 | 4 | 4 | 12, 24, 72, 108 | 0.22 ± 0.03 | Plutea_AminoType17 |
| 114 | 1 | 3 | 72 | 1.38 | Plutea_AminoType4 |
| 115 | 2 | 2,3 | 4 (2); 72 (3) | 0.68 | Plutea_AminoType21 |
| 117 | 1 | 2 | 108 | 0.38 | Plutea_AminoType21 |
| 118 | 1 | 4 | 4 | 0.38 | Plutea_AminoType10 |
| Control treatment | |||||
| 91 | 1 | 2 | 72 | 0.02 | Plutea_AminoType4 |
“Aminotype ID” indicates the name of the aminotype. “N_Observations” lists the total number of fragments in which a given aminotype was observed. “Colony” indicates the coral colony IDs in which a given aminotype was detected. “Time observed (h)” lists the sampling timepoints at which an aminotype was detected; numbers in parentheses indicate in which colonies each aminotype was observed at a given timepoint. “Mean relative abundance (%)” indicates the mean relative abundance of an aminotype across the fragments from which it was detected; standard deviations (SD) are given when aminotypes were identified in three or more samples. “Closest match” lists the strongest similarity of an aminotype in this study to known or putative viruses based on BLASTx against a reference database containing all sequences from the Reference Viral DataBase [63] and aminotypes generated by reprocessing the data in [29]. Colonies 1, 4, and 5 are Pocillopora verrucosa harboring Cladocopium pacificum; colonies 2 and 3 are Pocillopora ligulata harboring Cladocopium latusorum.