Fig. 1.

Immunohistochemical expression of BRAFV600E in odontogenic lesions. a Peripheral/Extraosseous Ameloblastoma: intense expression of BRAF V600E mutation, both in the follicular and in the plexiform pattern (×2 magnification). The mutation extension involves 30%–40% of the tumoral cells (n. 42, Supplementary Table 1). b The inset area of greater magnification (×20) shows the cytoplasmic expression of BRAF V600E both in the peripheral layer and in the central cells of neoplastic nests and strands. c Unicystic Ameloblastoma: moderate intensity and high extension (90%) of BRAF V600E mutation in plexiform pattern (×10 magnification), (n. 7, Supplementary Table 1). d The inset area of greater magnification (×20) shows the cytoplasmic expression of BRAF V600E in the ameloblastic epithelium islands. e Unicystic Ameloblastoma: moderate intensity and high extension (70%–80%) of BRAF V600E mutation in follicular pattern (×10 magnification), (n. 3, Supplementary Table 1). f The inset area of greater magnification (×20) shows the cytoplasmic expression of BRAF V600E both in the peripheral layer and in the central neoplastic cells. g Conventional Ameloblastoma: absence of BRAF V600E expression (<2%) both in plexiform and in follicular pattern (×10 magnification), (n. 25, Supplementary Table 1). h The inset area of greater magnification (×20) shows no BRAF V600E expression