Fig. 5.

Effect of a supercritical CO₂ extract of Aloysia gratissima leaves (EAG) on the formalin test. Nociceptive behavior was considered as the time (s) of elevation, biting or licking of the paw in the first phase (A: 0–5 min) and second phase (B: 15–30 min) of the test. Mice (n = 6 per group) were treated with vehicle (V: NaCl 0.9% + 1% tween 80, 10 ml/kg), Indomethacin (INDO: 10 mg/kg) or EAG (10 mg/kg) 1 h prior to administration of formalin 2% i.pl. One-way ANOVA followed by Student-Newman-Keuls: ∗p < 0.05; ∗∗p < 0.01 and ∗∗∗p < 0.001 are different from the vehicle group. Results expressed as mean ± S.E.M.