FIGURE 2.

Evaluation of ddPCR outputs for different reaction conditions. Plots (A,B) represent the dependence of the outputs of the H3F3A K27M mutant and H3F3A wild-type targets (for the samples with and without mutation, respectively) on the preamplification cycle number with 50 copies of DNA target at the start of the reaction. Plot (C) represents the dependence of the yields of wild-type target on the number of DNA copies at the start of preamplification (eight cycles). The dotted lines are estimated trends, R ² are correlation coefficients.