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. 2022 Apr 12;10:831332. doi: 10.3389/fbioe.2022.831332

FIGURE 2.

FIGURE 2

Identifying the sensitivity and specificity of PCR-CRISPR detection and comparing sensitivity with other nucleic acid detection tools. (A) Sensitivity detection of wild and mutant templates carried out using PCR-CRISPR and M-crRNA-1. (B) Detection analysis of mutant RNA dilution series with RT-qPCR with a commercial kit. (C) PCR-CRISPR can discriminate different viral mutant or deletion sites. (D) PCR-CRISPR achieves specific detection of different viral RNA. (n = 3 technical replicates, two-tailed Student t-test; ***, p < 0.001; ****, p < 0.0001; bars represent mean ± s.e.m.).