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. 2022 Apr 12;10:831332. doi: 10.3389/fbioe.2022.831332

FIGURE 3.

FIGURE 3

The PCR-CRISPR method detects mixed mutant genes of low proportion. (A) Schematic of PCR-CRISPR detection of mutant RNA on a background of wild-type RNA. (B) PCR-CRISPR detects mixed wild-type RNA of different proportions on a background of RNA mixture. (C) PCR-CRISPR detects mixed mutant RNA of different proportions on a background of RNA mixture. (D) Sequencing peak of Sanger sequencing for RNA mixtures of different proportions. (n = 3 technical replicates, two-tailed Student t-test; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, no significant difference; bars represent mean ± s.e.m.).