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. 2022 Jan 18;16(5):1409–1419. doi: 10.1038/s41396-022-01192-0

Fig. 3. Characteristics of diplonemids under different conditions.

Fig. 3

General morphology of D. japonicum (A, C) and R. humris (D, F) at 72 h and 120 h time points, respectively, by differential interference contrast (DIC) microscopy. Double arrowheads point to numerous food vacuoles filled with FLB (A, D), and arrowheads indicate storage vacuoles in the Hemi medium-grown cells (C, F). Epifluorescence microscopy of D. japonicum (B) and R. humris (E) showing DAPI-stained nucleus (blue) and food vacuoles (double arrowheads), containing FLB (green) at different stages of digestion. Scale bar: 2.5 µm. G A comparison of major morphometric characteristics (shown as the mean value ± SD and range of cell length, width, and cell volume) of the D. japonicum and R. humris from both culture conditions.