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. 2022 Jan 18;16(5):1409–1419. doi: 10.1038/s41396-022-01192-0

Fig. 5. Characterization of red autofluorescence of diplonemids.

Fig. 5

A Panels showing emergence and dynamics of red fluorescence signal emitted by D. japonicum and R. humris. Time intervals as in Fig. 2A. B Time-course changes in mean red autofluorescence intensity of D. japonicum and R. humris freshly subjected to either the Hemi medium (HEMI; solid line) or artificial seawater (ASW; dashed line). C Confocal microscopy images of live D. japonicum showing the presence of red autofluorescence (af) and its colocalization with lysosomes stained with green LysoTracker (lyso); the signal is absent in the ASW-starved cells. Nucleus was stained with NucBlue (nb). Ph, phase contrast. Scale bar: 1 µm. D Time-course changes in abundance of reactive oxygen species produced by D. japonicum grown on bacteria or medium.