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. 2022 Apr 25;13:2237. doi: 10.1038/s41467-022-29896-z

Fig. 6. GATA6 may serve as a potential host-directed therapeutic target.

Fig. 6

a Electrophoretic mobility shift assay (EMSA) of lysates from HEK293T cells overexpressing GATA6 with labeled oligonucleotides derived from the promoter region of ACE2 in the presence of various concentrations of Pyrrothiogatain (0–500 µM). b The effect of various concentrations of Pyrrothiogatain on Calu-3 cell proliferation was determined using the XTT assay and presented as a percentage of cell viability of untreated cells. Data were analyzed from three biologically independent experiments by one-way ANOVA with Brown-Forsythe test. c Calu-3 cells were treated with 500 uM Pyrrothiogatain and the expression of GATA6 and ACE2 were analyzed by western blot at the indicated time. d Real-time PCR quantification of WT-SARS-CoV-2 in Calu-3 cells that were pretreated with 500 uM Pyrrothiogatain. Cells were infected using MOI = 0.002 for 48 h. Data were analyzed from two biologically independent experiments by two-tailed student’s t-test. Shown are means ± SD. **p < 0.01. e Calu-3 cells pretreated with 500 uM Pyrrothiogatain and infected with WT-SARS-CoV-2. Cells were fixed 24 h post-infection and stained with antisera against SARS-CoV-2 (green) and DAPI (blue). Scale bar, 20 mm. f mRNA read densities (reads per kilobase million, RPKM) of the six members of the GATA family in uninfected Calu-3 (red), Vero-E6 (green), and A549 cells (blue)66–68. Representative images of three biologically independent experiments are shown. Source data are provided as a Source Data file.