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. 2022 Apr 25;8:226. doi: 10.1038/s41420-022-00971-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Heatmap of significantly differently expressed genes in ONFH samples of the GSE74089 dataset. Each row represents a differentially expressed gene, while each column represents a sample; B volcanic map of significantly differentially expressed genes in ONFH samples of GSE74089 dataset. Red represents upregulated genes, while green represents down-regulated genes, |logFC | > 2, p < 0.01; C the Venn diagram of intersection among significantly upregulated genes from the GSE74089 dataset, the top 500 target genes by miR-21-5p predicted by the starBase database and the top 100 target genes predicted by the TargetScan and miRDB databases; D SOX5 expression predicted in the GSE74089 dataset. Red box indicates ONFH samples, while gray box indicates normal samples; E SOX5 mRNA expression in the cartilage tissues of patients with ONFH (n = 20) and patients with femoral neck fracture (normal control, n = 20) determined with RT-qPCR; F the binding site of miR-21-5p and SOX5 predicted with TargetScan; G the targeting relationship between miR-21-5p and SOX5 verified with dual-luciferase reporter gene assay; H the expression of miR-21-5p and SOX5 in hFOB1.19 cells and HUVECs determined with RT-qPCR. Cell experiment was repeated three times independently. *p < 0.05.