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. 2021 Dec 13;16(5):1275–1283. doi: 10.1038/s41396-021-01155-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Scheme of the microcosm setup specifying microbial inoculation points and the spatial arrangement and dimensions of agar patches A, B, and C. b Experimental scenarios and observed results. Upper left panel: T4 or ΔfilM do not disperse along hyphae over the air-gap. Lower left panel: ΔfilM does not transport T4 along hyphae. Upper right panel: WT disperse along hyphae. Lower right panel: WT disperses along hyphae and transports T4 along hyphae over the air-gap. c, d T4 counts on agar patch A after 1 day in the absence and presence of ΔfilM (c) and WT (d). The solid and dashed lines indicate the median of 5 replicates and its 95CI of the initial inocula. e Time-dependent cumulative transport rates of WT (R_WT, in green) and phages (R_T4, in gray) to agar patches B and C (cf. Eq. 1). f Time-dependent phage transport efficiency in presence of WT (E_T4). g Time-dependent phage transport capacity of WT (C_T4). Data on T4 transport by ΔfilM are not shown as no transport was observed. Notches of the boxes represent 95CI of 5 replicates. If notches of two conditions do not overlap, it indicates a statistical difference between the two conditions.