Table 1.
Effect of Kupchan fractions on cell viability of T. brucei, Chang and Jurkat cells.
| FRACTIONS | MEAN IC50 ± SEM (μg/mL) |
SI |
|||
|---|---|---|---|---|---|
| T. brucei | Jurkat | Chang liver | Jurkat | Chang liver | |
| BPC | 6.72 ± 0.04 | NA | NA | NA | NA |
| BPFD | 3.29 ± 0.03 | 9.57 ± 0.10 | 115.45 ± 0.05 | 2.91 | 35.09 |
| BPFH | 6.64 ± 0.05 | NA | NA | NA | NA |
| BPFM | 5.86 ± 0.04 | 16.52 ± 0.03 | 147.10 ± 0.03 | 2.82 | 25.10 |
| BPWB | 12.97 ± 0.12 | NA | NA | NA | NA |
| DA | 0.57 ± 0.15 | NA | 37.52 ± 0.02 | NA | 65.83 |
| DX | NA | 0.30 ± 0.25 | 141.52 ± 0.03 | NA | NA |
Mean IC50 values and standard errors of the mean (SEM) were calculated from three distinct experiments. SI was calculated as the ratio of IC50 in Jurkat or Chang liver cells to the IC50 in T. brucei. BP= B. pilosa L., whole plant; BPC = crude extract of BP; FD = Dichloromethane fraction; FM = methanol fraction; WB = water-butanol fraction; FH = hexane fraction; DA = Diminazene aceturate, (antitrypanosomal drug); DX = Doxorubicin, (antileukaemia drug); SI = selectivity index; NA=Not applicable (based on activities against T. brucei, only BPFD and BPFM were further investigated for their effects on Chang and Jurkat cells); DA and DX were used as positive controls.